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cd36‐deficient mice (Jackson Laboratory)

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Jackson Laboratory cd36‐deficient mice

EP 80317 reduces systemic and lung homogenate ROS and inflammatory mediators. (A) Study design. (B) Bar graphs and dot plots represent the relative expression of Igf1 mRNA in lung tissue of <t>CD36</t> +/+ and (F) CD36 ‐/‐ mice. (C) Mean plasma MDA levels of CD36 +/+ and (G) CD36 ‐/‐ mice, expressed as bar graphs and dot plots. (D) Bar graphs and dot plots represent the relative expression of Nox2 mRNA in lung tissue of CD36 +/+ and (H) CD36 ‐/‐ mice. (E) Bar graphs and dot plots of the total leucocytes recruitment in lung tissue by MPO assay of CD36 +/+ and (I) CD36 ‐/‐ mice. (J) Bar graphs and dot plots represent the mononuclear cells count per mm 2 of photomicrographs of lung tissue of CD36 +/+ and (K) CD36 ‐/‐ . (L) Representative photomicrographs of lungs after staining with hematoxylin‐eosin (scale bar: 100 μm). Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 6–11 per group for CD36 +/+ and n = 6 per group for CD36 ‐/‐ .

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1) Product Images from "Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice"

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

Journal: Journal of Biochemical and Molecular Toxicology

doi: 10.1002/jbt.70057

Figure Legend Snippet: EP 80317 reduces systemic and lung homogenate ROS and inflammatory mediators. (A) Study design. (B) Bar graphs and dot plots represent the relative expression of Igf1 mRNA in lung tissue of CD36 +/+ and (F) CD36 ‐/‐ mice. (C) Mean plasma MDA levels of CD36 +/+ and (G) CD36 ‐/‐ mice, expressed as bar graphs and dot plots. (D) Bar graphs and dot plots represent the relative expression of Nox2 mRNA in lung tissue of CD36 +/+ and (H) CD36 ‐/‐ mice. (E) Bar graphs and dot plots of the total leucocytes recruitment in lung tissue by MPO assay of CD36 +/+ and (I) CD36 ‐/‐ mice. (J) Bar graphs and dot plots represent the mononuclear cells count per mm 2 of photomicrographs of lung tissue of CD36 +/+ and (K) CD36 ‐/‐ . (L) Representative photomicrographs of lungs after staining with hematoxylin‐eosin (scale bar: 100 μm). Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 6–11 per group for CD36 +/+ and n = 6 per group for CD36 ‐/‐ .

Techniques Used: Expressing, Clinical Proteomics, MPO Assay, Staining

EP 80317 reduces lung NLRP3 inflammasome and pro‐inflammatory cytokines and chemokines. Bar graphs and dot plots represent the relative expression of (A) Nlrp3 , (B) Il1β , and (C) Il18 mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ mice. (D) Mean IL‐1β levels in lung homogenates of CD36 +/+ mice, expressed as bar graph and dot plot. Bar graphs and dot plots represent the relative expression of (E) Nfκb1 , (F) Nfκb2 , (G) RelA , (H) RelB , (I) Tnf , (J) Il6 , (K) Ccl2 , (L) Atx , (M) Cxcl1 , (N) Siglecf , and (O) Cd11c mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ . Data are mean ± SEM. * p < 0.05, ** p < 0.01, and ***p < 0.001, as assessed by an unpaired t test. n = 11 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Figure Legend Snippet: EP 80317 reduces lung NLRP3 inflammasome and pro‐inflammatory cytokines and chemokines. Bar graphs and dot plots represent the relative expression of (A) Nlrp3 , (B) Il1β , and (C) Il18 mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ mice. (D) Mean IL‐1β levels in lung homogenates of CD36 +/+ mice, expressed as bar graph and dot plot. Bar graphs and dot plots represent the relative expression of (E) Nfκb1 , (F) Nfκb2 , (G) RelA , (H) RelB , (I) Tnf , (J) Il6 , (K) Ccl2 , (L) Atx , (M) Cxcl1 , (N) Siglecf , and (O) Cd11c mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ . Data are mean ± SEM. * p < 0.05, ** p < 0.01, and ***p < 0.001, as assessed by an unpaired t test. n = 11 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques Used: Expressing

EP 80317 decreases arachidonic acid metabolites‐PGE 2 . PGE 2 levels in lung homogenates of (A) CD36 +/+ and (B) CD36 ‐/‐ mice, represented as bar graphs. Bar graphs and dot plots represent the relative expression of Ptges mRNA in lung tissue of (C) CD36 +/+ and (D) CD36 ‐/‐ mice. (E) Bar graph and dot plot represent the relative expression of Cox2 mRNA in lung tissue of CD36 +/+ . Relative expression of (F) Ptger2 , (G) Ptger4 , and (H) Ptger3 mRNA in lung tissue of CD36 +/+ mice, expressed as bar graphs and dot plots. Bar graphs and dot plots represent the relative expression of (I) Ptger2 and (J) Ptger4 mRNA in lung tissue of CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Figure Legend Snippet: EP 80317 decreases arachidonic acid metabolites‐PGE 2 . PGE 2 levels in lung homogenates of (A) CD36 +/+ and (B) CD36 ‐/‐ mice, represented as bar graphs. Bar graphs and dot plots represent the relative expression of Ptges mRNA in lung tissue of (C) CD36 +/+ and (D) CD36 ‐/‐ mice. (E) Bar graph and dot plot represent the relative expression of Cox2 mRNA in lung tissue of CD36 +/+ . Relative expression of (F) Ptger2 , (G) Ptger4 , and (H) Ptger3 mRNA in lung tissue of CD36 +/+ mice, expressed as bar graphs and dot plots. Bar graphs and dot plots represent the relative expression of (I) Ptger2 and (J) Ptger4 mRNA in lung tissue of CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques Used: Expressing

EP 80317 decreases arachidonic acid metabolites‐LTB 4 . (A) Bar graph and dot plot represent the LTB 4 levels in lung homogenates of CD36 +/+ and (B) CD36 ‐/‐ mice. (C) Relative expression of Alox5 mRNA in lung tissue of CD36 +/+ and (D) CD36 ‐/‐ mice. Bar graph and dot plot represent the relative expression of (E) Alox12 , (F) Alox15 and (G) Alox5ap mRNA in lung tissue of CD36 +/+ mice. (H) Bar graph and dot plot of the relative expression of Ltc4s mRNA in lung tissue of CD36 +/+ . (I) Bar graph and dot plot represent the relative expression of Ltb4r1 mRNA in lung tissue of CD36 +/+ and (J) CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Figure Legend Snippet: EP 80317 decreases arachidonic acid metabolites‐LTB 4 . (A) Bar graph and dot plot represent the LTB 4 levels in lung homogenates of CD36 +/+ and (B) CD36 ‐/‐ mice. (C) Relative expression of Alox5 mRNA in lung tissue of CD36 +/+ and (D) CD36 ‐/‐ mice. Bar graph and dot plot represent the relative expression of (E) Alox12 , (F) Alox15 and (G) Alox5ap mRNA in lung tissue of CD36 +/+ mice. (H) Bar graph and dot plot of the relative expression of Ltc4s mRNA in lung tissue of CD36 +/+ . (I) Bar graph and dot plot represent the relative expression of Ltb4r1 mRNA in lung tissue of CD36 +/+ and (J) CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques Used: Expressing

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Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 reduces systemic and lung homogenate ROS and inflammatory mediators. (A) Study design. (B) Bar graphs and dot plots represent the relative expression of Igf1 mRNA in lung tissue of CD36 +/+ and (F) CD36 ‐/‐ mice. (C) Mean plasma MDA levels of CD36 +/+ and (G) CD36 ‐/‐ mice, expressed as bar graphs and dot plots. (D) Bar graphs and dot plots represent the relative expression of Nox2 mRNA in lung tissue of CD36 +/+ and (H) CD36 ‐/‐ mice. (E) Bar graphs and dot plots of the total leucocytes recruitment in lung tissue by MPO assay of CD36 +/+ and (I) CD36 ‐/‐ mice. (J) Bar graphs and dot plots represent the mononuclear cells count per mm 2 of photomicrographs of lung tissue of CD36 +/+ and (K) CD36 ‐/‐ . (L) Representative photomicrographs of lungs after staining with hematoxylin‐eosin (scale bar: 100 μm). Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 6–11 per group for CD36 +/+ and n = 6 per group for CD36 ‐/‐ .

Article Snippet: CD36‐deficient (CD36 −/− ) mice and wild‐type littermate controls on a C57Bl/6 J background were obtained from Jackson Laboratories (Bar Harbor, Maine, USA) and bred following established protocols.

Techniques: Expressing, Clinical Proteomics, MPO Assay, Staining

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 reduces lung NLRP3 inflammasome and pro‐inflammatory cytokines and chemokines. Bar graphs and dot plots represent the relative expression of (A) Nlrp3 , (B) Il1β , and (C) Il18 mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ mice. (D) Mean IL‐1β levels in lung homogenates of CD36 +/+ mice, expressed as bar graph and dot plot. Bar graphs and dot plots represent the relative expression of (E) Nfκb1 , (F) Nfκb2 , (G) RelA , (H) RelB , (I) Tnf , (J) Il6 , (K) Ccl2 , (L) Atx , (M) Cxcl1 , (N) Siglecf , and (O) Cd11c mRNA in lung tissue of CD36 +/+ and CD36 ‐/‐ . Data are mean ± SEM. * p < 0.05, ** p < 0.01, and ***p < 0.001, as assessed by an unpaired t test. n = 11 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques: Expressing

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 decreases arachidonic acid metabolites‐PGE 2 . PGE 2 levels in lung homogenates of (A) CD36 +/+ and (B) CD36 ‐/‐ mice, represented as bar graphs. Bar graphs and dot plots represent the relative expression of Ptges mRNA in lung tissue of (C) CD36 +/+ and (D) CD36 ‐/‐ mice. (E) Bar graph and dot plot represent the relative expression of Cox2 mRNA in lung tissue of CD36 +/+ . Relative expression of (F) Ptger2 , (G) Ptger4 , and (H) Ptger3 mRNA in lung tissue of CD36 +/+ mice, expressed as bar graphs and dot plots. Bar graphs and dot plots represent the relative expression of (I) Ptger2 and (J) Ptger4 mRNA in lung tissue of CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques: Expressing

Journal: Journal of Biochemical and Molecular Toxicology

Article Title: Targeting CD36 With EP 80317 Reduces Remote Inflammatory Response to Hind Limb Ischemia‐Reperfusion in Mice

doi: 10.1002/jbt.70057

Figure Lengend Snippet: EP 80317 decreases arachidonic acid metabolites‐LTB 4 . (A) Bar graph and dot plot represent the LTB 4 levels in lung homogenates of CD36 +/+ and (B) CD36 ‐/‐ mice. (C) Relative expression of Alox5 mRNA in lung tissue of CD36 +/+ and (D) CD36 ‐/‐ mice. Bar graph and dot plot represent the relative expression of (E) Alox12 , (F) Alox15 and (G) Alox5ap mRNA in lung tissue of CD36 +/+ mice. (H) Bar graph and dot plot of the relative expression of Ltc4s mRNA in lung tissue of CD36 +/+ . (I) Bar graph and dot plot represent the relative expression of Ltb4r1 mRNA in lung tissue of CD36 +/+ and (J) CD36 ‐/‐ mice. Data are mean ± SEM. * p < 0.05 and *** p < 0.001, as assessed by an unpaired t test. n = 14 for CD36 +/+ and n = 6 for CD36 ‐/‐ .

Techniques: Expressing

NanoSIMS analyses of 2H− ions in the heart after administering an intravenous injection of 200 µl of 2H-TRLs to 4-month-old female wild-type (Cd36+/+) and Cd36−/− mice. 1.5 or 2 min after the injection, hearts were harvested and sections were prepared for NanoSIMS. Differences in quantitative NanoSIMS data were analyzed with a Student’s t test with Welch’s correction. Four mice were used in this experiment, one for each time point.

Journal: Cell metabolism

Article Title: NanoSIMS Analysis of Intravascular Lipolysis and Lipid Movement Across Capillaries and into Cardiomyocytes

doi: 10.1016/j.cmet.2018.03.017

Figure Lengend Snippet: NanoSIMS analyses of 2H− ions in the heart after administering an intravenous injection of 200 µl of 2H-TRLs to 4-month-old female wild-type (Cd36+/+) and Cd36−/− mice. 1.5 or 2 min after the injection, hearts were harvested and sections were prepared for NanoSIMS. Differences in quantitative NanoSIMS data were analyzed with a Student’s t test with Welch’s correction. Four mice were used in this experiment, one for each time point.

Article Snippet: Cd36 -deficient mice ( Cd36 −/− ) ( Febbraio et al., 1999 ) and wild-type mice were obtained from The Jackson Laboratory (strain C57BL/6).

Techniques: Injection

Effect of CD36 on H2O2-induced increase in intracellular calcium concentration ([Ca2+]i) in mouse lung microvascular endothelial cells (MLMVEC). A: scatterplot and mean ± SE of baseline intracellular calcium ([Ca2+]i) in wild-type (WT) and CD36-deficient (CD36−/−) MLMVEC. Representative traces from WT (B) and CD36−/− (C) MLMVEC show change in [Ca2+]i following exposure to 1 mM H2O2. D: scatterplot and mean ± SE values for peak change in [Ca2+]i from baseline in MLMVEC exposed to 1 mM H2O2. *Significant difference from WT; n = 5–13 experiments/group.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Effect of CD36 on H2O2-induced increase in intracellular calcium concentration ([Ca2+]i) in mouse lung microvascular endothelial cells (MLMVEC). A: scatterplot and mean ± SE of baseline intracellular calcium ([Ca2+]i) in wild-type (WT) and CD36-deficient (CD36−/−) MLMVEC. Representative traces from WT (B) and CD36−/− (C) MLMVEC show change in [Ca2+]i following exposure to 1 mM H2O2. D: scatterplot and mean ± SE values for peak change in [Ca2+]i from baseline in MLMVEC exposed to 1 mM H2O2. *Significant difference from WT; n = 5–13 experiments/group.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques: Concentration Assay

Transient receptor potential-vanilloid 4 (TRPV4) calcium channel expression and function in WT, CD36−/−, and Fyn-deficient (Fyn−/−) MLMVEC. A: immunoblot images showing expression of TRPV4 in WT, CD36−/−, and Fyn−/− MLMVEC. B: bar graph showing mean ± SE fold change in TRPV4 expression in WT, CD36−/−, and Fyn−/− MLMVEC lysates. Representative traces show change in [Ca2+]i in WT (C), CD36−/− (D), and Fyn−/− (E) MLMVEC following exposure to the TRPV4 agonist GSK 1016790A (GSK; 1.5 µM). F: scatterplot and mean ± SE change in peak [Ca2+]i from in WT, CD36−/−, and Fyn−/− MLMVEC following exposure to GSK. *Significant difference from WT; n = 6–8 experiments/group.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Transient receptor potential-vanilloid 4 (TRPV4) calcium channel expression and function in WT, CD36−/−, and Fyn-deficient (Fyn−/−) MLMVEC. A: immunoblot images showing expression of TRPV4 in WT, CD36−/−, and Fyn−/− MLMVEC. B: bar graph showing mean ± SE fold change in TRPV4 expression in WT, CD36−/−, and Fyn−/− MLMVEC lysates. Representative traces show change in [Ca2+]i in WT (C), CD36−/− (D), and Fyn−/− (E) MLMVEC following exposure to the TRPV4 agonist GSK 1016790A (GSK; 1.5 µM). F: scatterplot and mean ± SE change in peak [Ca2+]i from in WT, CD36−/−, and Fyn−/− MLMVEC following exposure to GSK. *Significant difference from WT; n = 6–8 experiments/group.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques: Expressing, Western Blot

TRPV4 mediated barrier dysfunction in WT, CD36−/−, and Fyn−/− MLMVEC. A: representative traces of transmembrane electrical resistance (TER) measurements normalized to baseline in WT, CD36−/−, and Fyn−/− MLMVEC following exposure to GSK1016790 (GSK; 1.5 µM). Scatterplots showing maximum decrease in TER (B) and area under the curve (C) for WT, CD36−/−, and Fyn−/− MLMVEC. *Significant difference from WT; n = 7–12 experiments/group.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: TRPV4 mediated barrier dysfunction in WT, CD36−/−, and Fyn−/− MLMVEC. A: representative traces of transmembrane electrical resistance (TER) measurements normalized to baseline in WT, CD36−/−, and Fyn−/− MLMVEC following exposure to GSK1016790 (GSK; 1.5 µM). Scatterplots showing maximum decrease in TER (B) and area under the curve (C) for WT, CD36−/−, and Fyn−/− MLMVEC. *Significant difference from WT; n = 7–12 experiments/group.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques:

$Subcellular localization of Fyn in CD36−/− MLMVEC. Representative immunofluorescence images of WT (A), CD36−/− (B), and SSO-treated WT (C) MLMVEC stained with anti-Fyn antibody (green) and nuclear counterstain (DAPI; blue). D: representative histograms showing distance of fluorescent pixels from the center of the cell nucleus in WT CD36−/− and SSO-treated MLMVEC. E: scatterplot showing mean ± SE distance from nucleus in WT, SSO-treated, and CD36−/− MLMVEC for the histogram shown in D. F: scatterplot showing mean ± SE perinuclear fraction in WT, SSO-treated, and CD36−/− MLMVEC. *Significant difference from WT; n = 3 independent experiments, consisting of 7–12 images for each condition.$

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Subcellular localization of Fyn in CD36−/− MLMVEC. Representative immunofluorescence images of WT (A), CD36−/− (B), and SSO-treated WT (C) MLMVEC stained with anti-Fyn antibody (green) and nuclear counterstain (DAPI; blue). D: representative histograms showing distance of fluorescent pixels from the center of the cell nucleus in WT CD36−/− and SSO-treated MLMVEC. E: scatterplot showing mean ± SE distance from nucleus in WT, SSO-treated, and CD36−/− MLMVEC for the histogram shown in D. F: scatterplot showing mean ± SE perinuclear fraction in WT, SSO-treated, and CD36−/− MLMVEC. *Significant difference from WT; n = 3 independent experiments, consisting of 7–12 images for each condition.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques: Immunofluorescence, Staining

Interactions between CD36, Fyn, and TRPV4. A: representative immunoblot (IB) images showing TRPV4 and housekeeping control (GAPDH) expression in WT and TRPV4−/− MLMVEC lysates. B: immunoblot images showing TRPV4 and Fyn expression in WT and CD36−/− MLMVEC lysates before (input) and after immunoprecipitation with either anti-Fyn (IP: Fyn) or IgG control (IP: IgG) antibody. C: immunoblot images showing TRPV4 expression in WT and CD36−/− MLMVEC lysates before (input) and after immunoprecipitation with either anti-phosphotyrosine (IP: P-Tyr) or IgG control (IP: IgG) antibody. D: densitometry scatterplot showing fold change in TRPV4 in Fyn (IP: Fyn) and phosphotyrosine (IP: pTyr) immunoprecipitate lysates. *Significant difference from WT; n = 3 experiments/group.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Interactions between CD36, Fyn, and TRPV4. A: representative immunoblot (IB) images showing TRPV4 and housekeeping control (GAPDH) expression in WT and TRPV4−/− MLMVEC lysates. B: immunoblot images showing TRPV4 and Fyn expression in WT and CD36−/− MLMVEC lysates before (input) and after immunoprecipitation with either anti-Fyn (IP: Fyn) or IgG control (IP: IgG) antibody. C: immunoblot images showing TRPV4 expression in WT and CD36−/− MLMVEC lysates before (input) and after immunoprecipitation with either anti-phosphotyrosine (IP: P-Tyr) or IgG control (IP: IgG) antibody. D: densitometry scatterplot showing fold change in TRPV4 in Fyn (IP: Fyn) and phosphotyrosine (IP: pTyr) immunoprecipitate lysates. *Significant difference from WT; n = 3 experiments/group.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques: Western Blot, Control, Expressing, Immunoprecipitation

Effect of SSO on AcLDL uptake in MLMVEC. Representative immunofluorescence images of untreated (A) and SSO-treated (100 µM, 1 h; B) WT MLMVEC or CD36−/− MLMVEC. All cells were stained for AcLDL uptake (Alexa Fluor 598; red) and nuclear counterstain (DAPI; blue). D: scatterplot showing mean ± SE values for number of cells staining positive for AcLDL in WT, SSO-treated, and CD36−/− MLMVEC. *Significant difference from control; n = 5 images/group from at least 3 independent experiments.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Effect of SSO on AcLDL uptake in MLMVEC. Representative immunofluorescence images of untreated (A) and SSO-treated (100 µM, 1 h; B) WT MLMVEC or CD36−/− MLMVEC. All cells were stained for AcLDL uptake (Alexa Fluor 598; red) and nuclear counterstain (DAPI; blue). D: scatterplot showing mean ± SE values for number of cells staining positive for AcLDL in WT, SSO-treated, and CD36−/− MLMVEC. *Significant difference from control; n = 5 images/group from at least 3 independent experiments.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques: Immunofluorescence, Staining, Control

Lung ischemia-reperfusion (IR) injury is attenuated in CD36−/− mice. Left lung/right lung Evans blue dye (EBD) ratio in WT and CD36−/− mice following sham or IR surgery; n = 4–6 mice per group, *Significant difference from WT Sham; **Significant difference from WT IR.

Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

Article Title: CD36 mediates H 2 O 2 -induced calcium influx in lung microvascular endothelial cells

doi: 10.1152/ajplung.00361.2016

Figure Lengend Snippet: Lung ischemia-reperfusion (IR) injury is attenuated in CD36−/− mice. Left lung/right lung Evans blue dye (EBD) ratio in WT and CD36−/− mice following sham or IR surgery; n = 4–6 mice per group, *Significant difference from WT Sham; **Significant difference from WT IR.

Article Snippet: CD36-deficient C57/B6 ( CD36 −/− ) male mice were generously provided by Dr. Alan Scott (Johns Hopkins University).

Techniques:

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